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By Thomas G. , Ii & Theresa P. Pretlow (editors) Pretlow

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In our hands, Ficoll adds less than 3 mosmoles/liter to the isokinetic gradient (Pretlow, 1971) we use for velocity sedimentation. There has been some controversy about the osmolarities and viscosities of solutions of albumin and Ficoll in some laboratories (reviewed in Pretlow et aL, 1975). Because of the frequency with which these media are used, it seems important to emphasize the fact that their physical properties become suboptimal when they are suboptimally prepared. The appropriate meth­ ods for the preparation of albumin (Kneece and Leif, 1971) and Ficoll (Pretlow et aL, 1975) have been described.

1978b). Isolation and characterization of the proteolytic enzyme component from commercially available crude trypsins. Anal. Biochem. 84, 2 0 5 - 2 1 7 . Steinberg, M. S. (1962). The role of temperature in the control of aggregation of dissociated embryonic cells. Exp. Cell Res. 28, 1-10. Steinberg, M. S. (1963). " E C M " : Its nature, origin and function in cell aggregation, Exp. Cell Res. 30, 2 5 7 - 2 7 9 . Sullivan, J. C , and Schäfer, I. A. (1966). Survival of pronase-treated cells in tissue culture.

Dickler, Η. Β . , Froland, S. , Fudenberg, H. H . , Greaves, M. P . , Grey, H. M . , Kunkel, Η. , Natvig, J. B . , Ritts, R. , Rowe, D. , Siegal, F. , Terry, W. D . , and Wybran, J. (1974). Identification, enumeration, and isolation of Β and Τ lymphocytes from human peripheral blood: Report of a WHO/IARC-sponsored workshop on human Β and Τ cells, London, 15-17 July 1974. Scand. J. Immunol. 3 , 5 2 1 - 5 3 2 . de Duve, C. (1971). Tissue fractionation, past and present. J. Cell Biol. 50, 2 0 D - 5 5 D .

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