Download Bioluminescence and Chemiluminescence Part B by Nathan P. Colowick, Nathan P. Kaplan, Marlene A. DeLuca, PDF

By Nathan P. Colowick, Nathan P. Kaplan, Marlene A. DeLuca, William D. McElroy

The severely acclaimed laboratory ordinary, Methods in Enzymology, is without doubt one of the so much hugely revered courses within the box of biochemistry. due to the fact that 1955, each one quantity has been eagerly awaited, often consulted, and praised by way of researchers and reviewers alike. The sequence includes a lot fabric nonetheless suitable this day - really a necessary booklet for researchers in all fields of existence sciences

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Gibbs, J. Biolumin. Chemilurnin. 1, 1 (1986). 6o N. A. Hendy and P. P. Gray, Biotechnol. Bioeng. 21, 153 (1979). 6~ T. S. Tsai and L. J. Everett, in "Analytical Applications of Bioluminescence and Chemiluminescence" (L. J. Kricka, P. E. Stanley, G. H. G. Thorpe, and T. P. ), p. 75. Academic Press, London, 1984. [2] EXTRACTIONOF ATP FROMCELLS 21 Somatic and Microbial Cells Mixed Together with Nonliving Material This is the most complex system. Examples in this section include soil, 4 nematodes in s o i l , 49 meat, 59 milk, 62 rumen contents, 12 fruit j u i c e s , 63 and clinical samples.

Below) and I ml 1M potassium acetate and was kept at room temperature during the assays. The reagent could be kept for several days in a refrigerator. 1 M tris(hydroxymethyl)aminomethane (E. Merck, Darmstadt, FRG) containing 2 mM EDTA (E. 75 with acetic acid. ATP Standard (LKB-Wallac) was reconstituted with 10 ml distilled water to give a final concentration of 10 /zM ATP. ) and pyruvate kinase (10 mg/ml) in 50% glycerol (Boehringer Mannheim, FRG). 0 with acetic acid before being mixed with pyruvate kinase.

Of the samples determined to be positive for significant bacteriurea (true 7 B. A. Hanna, L. Larrier, C. F. Fam, and A. C. Coxon, Pathologist (in press), 8 D. N. Alexander, G. M. Ederer, and J. M. Matsen, J. Clin. Microbiol. 3, 42 (1976). 9 G. Szilagyi, V. Aning, and A. Karman, J. Clin. Lab. Autom. 3, 117 (1983). 1) 77(55) 35(25) " At three different breakpoints for significant riurea (n = 140). b False positive, luciferase positive, colony negative. ' False negative, luciferase negative, colony positive.

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