By Lyn Healy, Ludmila Ruban
This lavishly-illustrated, authoritative atlas explores the tricky artwork of culturing human pluripotent stem cells. Twelve chapters – containing greater than 280 colour illustrations – hide various themes in pluripotent stem telephone culturing together with mouse and human fibroblasts, human embryonic stem cells and precipitated pluripotent stem cells, attribute staining styles, and irregular cultures, between others. Atlas of Human Pluripotent Stem Cells in Culture is a accomplished selection of illustrated thoughts complemented through informative and academic captions analyzing what quality cells seem like and the way they behave in quite a few environments. Examples of excellent cultures are in comparison side-by-side to less-than-perfect and unacceptable examples of human embryonic and caused pluripotent stem mobilephone colonies. This particular and thorough atlas is a useful source for researchers, academics, and scholars who're attracted to or operating with stem phone culturing.
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Additional info for Atlas of Human Pluripotent Stem Cells in Culture
2011;63:104–10. Eiselleova L, Peterkova I, Neradil J, Slaninova I, Hampl A, Dvorak P. Comparative study of mouse and human feeder cells for human embryonic stem cells. Int J Dev Biol. 2008;52:353–63. Fernandes AM, Meletti T, Guimarães R, Stelling MP, Marinho PA, Valladão AS, Rehen SK. Worldwide survey of published procedures to culture human embryonic stem cells. Cell Transplant. 2010;19:509–23. Lee JB, Song JM, Lee JE, Park JH, Kim SJ, Kang SM, et al. Available human feeder cells for the maintenance of human embryonic stem cells.
B) The large colony in the middle of the image is undifferentiated, but all of the smaller colonies in the image are too small to form any decent compacted colonies (both ×4 magnification) 44 4 Human Embryonic Stem Cells a b Fig. 18 (a) Darkfield microscopy of a good colony under a dissecting microscope. (b) The same colony with a little bit of illumination behind it. This is the preferable way of illuminating culture plates for mechanical passaging. Under these conditions, it is much easier to a recognise differentiation or areas of potential differentiation.
16 (a–d) Images of very low-density feeders, 24 h after plating. Note the large spaces between the cells and the emergence of large cells (black arrows), indicating that these cultures are not optimal. Inactivated Mouse and Human Fibroblasts In general, these cells would not support the growth and expansion of undifferentiated PSCs (All ×10 magnification) 3 Inactivated Mouse and Human Fibroblasts a 29 b Fig. 17 (a, b) Optimal density of good-quality mouse feeders. These cells would support the growth and expansion of undifferentiated PSCs (a, ×4 magnification; b, ×10) a b c Fig.