Download Animal Cell Electroporation and Electrofusion Protocols by Jac A. Nickoloff PDF

By Jac A. Nickoloff

This brilliant selection of well-tested protocols for the electroporation of proteins and DNA into insect, fish, and mammalian cells additionally comprises confirmed animal mobilephone electrofusion suggestions for stories of somatic mobile genetics and improvement, and for producing monoclonal antibodies. it truly is uncommon via its insurance of significant version phone varieties from many organisms and tissue forms and its unique directions for the expansion and training of particular cells to accomplish optimal transfection. Its vast reference lists, citations of different move tools, suggestion on pitfalls to prevent, and outlines of anticipated effects determine comfortably reproducible effects even for newbies.

Show description

Read or Download Animal Cell Electroporation and Electrofusion Protocols PDF

Best molecular biology books

Origin and Evolution of Telomeres

Telomerase: evolution, constitution, and serve as / Marie-Eve Brault, Yasmin D'Souza, and Chantal Autexier -- Drosophila telomeres: a version at the telomerase topic / Mary-Lou Pardue and P. Gregory DeBaryshe -- substitute lengthening of telomeres in mammalian cells / Anthony J. Cesare and Roger R. Reddel -- T-loops, T-circles, and slippery forks / Sarah A.

Stochastic Transport in Complex Systems: From Molecules to Vehicles

The 1st a part of the e-book presents a pedagogical advent to the physics of advanced platforms pushed faraway from equilibrium. during this half we talk about the fundamental suggestions and theoretical suggestions that are accepted to review classical stochastic shipping in platforms of interacting pushed debris.

Advanced Optical Flow Cytometry: Methods and Disease Diagnoses

A close examine the most recent examine in non-invasive in vivo cytometry and its functions, with specific emphasis on novel biophotonic equipment, disorder analysis, and tracking of affliction therapy at unmarried mobilephone point in desk bound and move stipulations. This ebook therefore covers the spectrum starting from basic interactions among mild, cells, vascular tissue, and phone labeling debris, to ideas and possibilities for preclinical and medical study.

Managing Salt Tolerance in Plants: Molecular and Genomic Perspectives

Salinity pressure presently affects greater than eighty million hectares of land around the globe and extra arable land might be impacted sooner or later as a result of worldwide weather adjustments. coping with Salt Tolerance in crops: Molecular and Genomic views provides specific molecular and genomic techniques for the improvement of crop crops tolerant to salinity rigidity.

Extra resources for Animal Cell Electroporation and Electrofusion Protocols

Example text

14. Xie, T. D. and Tsong, T Y. (1992) Study of mechanisms of electric field-induced DNA transfection III Electrrc parameters and other conditions for effective transfectlon. Biophys. J. 63,28-34. 15. Kubimec, R. , and Hui, S. W. (1990) Effects of pulse length and pulse strength on transfectlon by electroporatlon. BioTechniques 8, l-3. 16. Chang, D. , Chassy, B. , Saunders, J. , and Sowers, A. E. (1992) Gwdefor Electroporation and Electrojksion Academic, San Diego. 17. Baker, P. F. and Knight, D.

Generators with Other 5. Chambers There are many choices in chambers for ECM. In general, chambers need to create the required field strength from the voltage delivered to the electrodes by the generator; they need to contain the appropriate volume, and need to be sterilized or sterilizable, easily filled, emptied, and if reused, easily cleaned. Table 5 gives the main trade-off parameters in the selection of chambers. The following describes only the more frequently used chambers in the field. Instrumentation 51 Table 5 Chamber Trade-off Parameters Small volume Disposable Homogeneous field Visualization of cells Batch process Aluminum electrodes Presterilized Small gap Fig.

Notes 1. Electroporation can be used to mtroduce a variety of proteins mto cells, including monoclonal antibodies (MAb) (22,23), functional enzymes (24), other endonucleases in addition to restriction enzymes (25), and a host of other proteins and protein products (26-28). 2. Both the Bio-Rad Gene Pulser and the BRL Cell Porator are effective in the introduction of enzymes into the cell, but the most effective conditions for the BRL machine also result in relatively high levels of cell death (10).

Download PDF sample

Rated 4.61 of 5 – based on 12 votes