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By Daniel L. Purich

The Advances in Enzymology and similar parts of Molecular Biology Series is likely one of the such a lot prestigious within the box, dedicated to the newest tendencies in molecular biology and enzymology. every one quantity of the sequence comprises contributions from best gurus within the box. less than Dr. Purich's editorship, which started with quantity seventy two, the sequence has improved its assurance to incorporate thematic volumes targeting particular learn components, in addition to non-thematic volumes along with chapters with a extra common allure.

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Extra info for Advances in Enzymology and Related Areas of Molecular Biology, Mechanism of Enzyme Action

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Probable mechanisms of the formation of lactate containing fixed carbon have already been considered in the section on bacteria (page 166). A logical objection can be raised to the scheme in Fig. 4 which assigns an essential role to a fixation reaction solely on the basis of the presence and location of fixed carbon in a compound. Fairly reliable information on the mechanism of fixation can be obtained, but the answer as to whether or not the process as a whole is dependent on the fixation reaction cannot be obtained by this method.

More extensive studies are needed before a definite conclusion can be drawn, but at present the possibility of a general fixation of carbon dioxide through the action of carboxylases aa a group seems remote. Fixation by carboxylases may be limited largely to the enzyme studied by Knunpitr et al. Evans (61),likewise, has found no evidence of exchange during the action of carboxylase on pyruvate. The exchange was studied by use of radioactive carbon dioxide at pressures as high as 300 atmospheres.

It is evident that the observed fixation could have been due to urea formation. Therefore, the demonstration of fixation, unaccompanied by identification of the compound concerned, does not give reliable evidence for the suggested reactions. B. FIXATION OF CARBON DIOXIDE INVOLVJNG A CARBON TO CARBON LINKAQE 1. COOH The reader is referred to this discussion (p. 153) for a more detailed account concerning the above fixation reaction, for despite Krebs' statement, no direct evidence has been obtained as yet with animal tissue which permits the definite conclusion that pyruvic or oxalacetic acid is a component of the fixation reaction.

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