Download Advanced Optical Flow Cytometry: Methods and Disease by Valery V. Tuchin PDF

By Valery V. Tuchin

An in depth examine the most recent learn in non-invasive in vivo cytometry and its functions, with specific emphasis on novel biophotonic tools, illness analysis, and tracking of affliction therapy at unmarried mobilephone point in desk bound and circulate conditions.This booklet hence covers the spectrum starting from primary interactions among mild, cells, vascular tissue, and cellphone labeling debris, to suggestions and possibilities for preclinical and scientific examine. common issues contain gentle scattering by means of cells, quickly video microscopy, polarization, laser-scanning, fluorescence, Raman, multi-photon, photothermal, and photoacoustic equipment for mobile diagnostics and tracking of affliction therapy in dwelling organisms. additionally provided are discussions of complex tools and strategies of classical stream cytometry.

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Advanced Optical Flow Cytometry: Methods and Disease Diagnoses

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Spectrum of single molecules, therefore, cannot be detected. Surface enhanced raman scattering (SERS) can solve this problem and make this method sensitive enough for FCM [85]. Another vibrational spectroscopic technique, near infrared spectroscopy (NIRS), allows for noninvasive monitoring of oxygenation and perfusion [86–89], functional brain imaging [90], or examination of normal and malignant tissue, for example, applied for breast cancer imaging [91]. Although this method is mainly used for imaging in clinical applications, it might also be interesting for tissomics and cytometry, for example, for distinguishing intact cartilage from the enzymatically digested one [92] or the characterization, discrimination, and identification of microorganisms [93].

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B. (2001) Application of laser scanning cytometry for evaluation of DNA ploidy in routine cytologic specimens. Diagn. , 24 (3), 200–205. , and Ikeda, E. (1996) DNA ploidy analysis by laser scanning cytometry (lsc) in colorectal cancers and comparison with flow cytometry. Cytometry, 23 (2), 106–109. , and Zhao, H. (2008) Analysis of apoptosis by cytometry using tunel assay. Methods, 44 (3), 250–254. , and Gorczyca, W. (2000) Proliferation and apoptosis in solid tumors. Analysis by laser scanning cytometry.

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